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Lls have been incubated with nonenzymatic cell dissociation buffer at 37C for 10 min and had been scraped meticulously and replated in cAMP-Screen Direct precoated 96-well assay plates at a density of 1.0 10 five cells per effectively to assess cAMP production. Twenty-four hours later, the medium was re- placed with FBS-free medium for 2 h, followed by three h of LPS stimulation. The culture mediu

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